CRISPR Cas9-Mediated Selective Isothermal Amplification for Sensitive Detection of Rare Mutant Alleles

Junman Chen; Tian Qiu; Michael G Mauk; Yaguang Fan; Yong Jiang; Jianming Ying; Qinghua Zhou; Youlin Qiao; Haim H Bau; Jinzhao Song

doi:10.1093/clinchem/hvab163

CRISPR Cas9-Mediated Selective Isothermal Amplification for Sensitive Detection of Rare Mutant Alleles

Clin Chem. 2021 Nov 1;67(11):1569-1571. doi: 10.1093/clinchem/hvab163.

Authors

Junman Chen^{1

2}, Tian Qiu³, Michael G Mauk², Yaguang Fan⁴, Yong Jiang⁵, Jianming Ying³, Qinghua Zhou⁶, Youlin Qiao⁵, Haim H Bau², Jinzhao Song^{1

2}

Affiliations

¹ The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
² Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA.
³ Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
⁴ Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China.
⁵ Center for Global Health, School of Population Medicine and Public Health, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
⁶ Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Chengdu, Sichuan University, China.

No abstract available

Keywords: isothermal nucleic acids amplification; liquid biopsy; mutant allele enrichment; mutation detection; point-of-care testing; programmable endonuclease.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Alleles
CRISPR-Cas Systems*
Humans
Limit of Detection
Nucleic Acid Amplification Techniques*

Publication types

MeSH terms

Grants and funding