Detecting Persister Awakening Determinants

Dorien Wilmaerts; Jan Michiels; Natalie Verstraeten

doi:10.1007/978-1-0716-1621-5_13

Detecting Persister Awakening Determinants

Methods Mol Biol. 2021:2357:197-208. doi: 10.1007/978-1-0716-1621-5_13.

Authors

Dorien Wilmaerts^{1

2}, Jan Michiels^{3

4}, Natalie Verstraeten^{1

2}

Affiliations

¹ VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium.
² Centre of Microbial and Plant Genetics, KU Leuven, Leuven, Belgium.
³ VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium. jan.michiels@kuleuven.be.
⁴ Centre of Microbial and Plant Genetics, KU Leuven, Leuven, Belgium. jan.michiels@kuleuven.be.

PMID: 34590260
DOI: 10.1007/978-1-0716-1621-5_13

Abstract

For long, persistence research has focused primarily on disentangling mechanisms of persister state entry. Due to the rapid advances in the field of single-cell techniques and newly obtained insights in the persister phenotype, studying persister awakening has been unlocked and it has gained much interest in the scientific community. However, a framework on how this research should be conducted is currently lacking. Therefore, we here present a method to detect and validate genes important for persister awakening.

Keywords: Cell sorting; Persister; Persister awakening; Single-cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Wakefulness*

Substances

Anti-Bacterial Agents