Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa

María López Chiloeches; Anna Bergonzini; Teresa Frisan; Océane C B Martin

doi:10.1016/j.xpro.2021.100833

Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa

STAR Protoc. 2021 Sep 15;2(4):100833. doi: 10.1016/j.xpro.2021.100833. eCollection 2021 Dec 17.

Authors

María López Chiloeches^{1

2}, Anna Bergonzini^{1

2}, Teresa Frisan^{1

2}, Océane C B Martin³

Affiliations

¹ Department of Molecular Biology, Umeå University, 90736 Umeå, Sweden.
² Umeå Centre for Microbial Research (UCMR), Umeå University, 90736 Umeå, Sweden.
³ Univ. Bordeaux, INSERM, BaRITOn, U1053, 33000 Bordeaux, France.

Abstract

We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe how to assess the extent and features of infiltrating macrophages by double immunofluorescence. Total macrophage population was determined using an F4/80 antibody, whereas the specific M2-like population was assessed using a CD206 antibody. For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).

Keywords: Cell-based assays; In situ hybridization; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Colon
Fluorescent Antibody Technique
Intestinal Mucosa*
Macrophages*
Mice
Salmonella typhimurium