Protocol for Drosophila sleep deprivation using single-chip board

Xi Jin; Pengyu Gu; Junhai Han

doi:10.1016/j.xpro.2021.100827

Protocol for Drosophila sleep deprivation using single-chip board

STAR Protoc. 2021 Sep 17;2(4):100827. doi: 10.1016/j.xpro.2021.100827. eCollection 2021 Dec 17.

Authors

Xi Jin¹, Pengyu Gu¹, Junhai Han¹

Affiliation

¹ School of Life Science and Technology, the Key Laboratory of Developmental Genes and Human Disease, Southeast University, 2 Sipailou Road, Nanjing 210096, China.

Abstract

Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in Drosophila. This deprivation system is featured by its programming flexibility. The knowledge of electronic circuits and a certain level of programming are both required to fulfill this protocol. For complete details on the use and execution of this protocol, please refer to Jin et al. (2021).

Keywords: Behavior; Bioinformatics; Biophysics; Cognitive Neuroscience; Computer sciences; Model Organisms; Neuroscience; Physics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Behavior, Animal / physiology*
Behavioral Research* / instrumentation
Behavioral Research* / methods
Disease Models, Animal*
Drosophila / physiology*
Equipment Design
Female
Homeostasis / physiology
Male
Sleep Deprivation / physiopathology*