Wild mushrooms showed analgesic and cytotoxic properties along with phytoconstituent's binding affinity to COX-1, COX-2 and cytochrome P450 2C9

S M Moazzem Hossen; Mohammad Shahadat Hossain; Sumaiya Akbar; Umme Tahmida; Jannatul Mawa; Nazim Uddin Emon

doi:10.1016/j.heliyon.2021.e07997

Wild mushrooms showed analgesic and cytotoxic properties along with phytoconstituent's binding affinity to COX-1, COX-2 and cytochrome P450 2C9

Heliyon. 2021 Sep 14;7(9):e07997. doi: 10.1016/j.heliyon.2021.e07997. eCollection 2021 Sep.

Authors

S M Moazzem Hossen¹, Mohammad Shahadat Hossain¹, Sumaiya Akbar¹, Umme Tahmida¹, Jannatul Mawa¹, Nazim Uddin Emon²

Affiliations

¹ Department of Pharmacy, Faculty of Biological Science, University of Chittagong, Chittagong 4331, Bangladesh.
² Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong 4318, Bangladesh.

Abstract

This study was designed to evaluate the cytotoxic and analgesic potential of methanol extracts of five wild mushrooms available in the University of Chittagong, Bangladesh. The acetic acid-induced writhing method was used for the analgesic activity, while cytotoxicity was tested using brine shrimp lethality bioassay. In silico molecular docking and ADME/T study have been employed by using Schrodinger v11.1, BIOVIA Discovery Studio 2020 and online tool (AdmeSAR) respectively. The methanol extracts of Daldinia concentrica, Trametes lactinea, Fomitopsis cajanderi and Daedaleopsis confragosa exhibited a significant (p < 0.001) decrease in the number of writhing when compared to the control group. Except for Lentinus squarrosulus at 200 mg/kg body weight, the remaining mushroom extracts showed equal to or above 50 % inhibition of writhing. Daldinia concentrica showed the lowest LC₅₀ values with 0.63 μg/mL, while Daedaleopsis confragosa showed the highest LC₅₀ values of 2.33 μg/mL, indicating decisive cytotoxic action all mushrooms extracts. Considering the secondary metabolites, daldinan A and fomlactone A were found the most promising myco-compounds in analgesic and cytotoxic molecular docking studies. Besides, all the selected metabolites meet the rule of Lipinski's drug-likeliness. These results concluded that each mushroom extracts except Lentinus squarrosulus possess a potential analgesic. All the mushroom extracts embrace a promising cytotoxic activity that may guide the progress of a new drug.

Keywords: Analgesic; Cytotoxic; In silico; Molecular docking; Receptors; Wild mushrooms.