Identification and Pathogenicity Evaluation of a Novel Reassortant Infectious Bursal Disease Virus (Genotype A2dB3)

Yulong Wang; Nan Jiang; Linjin Fan; Xinxin Niu; Wenying Zhang; Mengmeng Huang; Li Gao; Kai Li; Yulong Gao; Changjun Liu; Hongyu Cui; Aijing Liu; Qing Pan; Yanping Zhang; Xiaomei Wang; Xiaole Qi

doi:10.3390/v13091682

Identification and Pathogenicity Evaluation of a Novel Reassortant Infectious Bursal Disease Virus (Genotype A2dB3)

Viruses. 2021 Aug 25;13(9):1682. doi: 10.3390/v13091682.

Authors

Yulong Wang^{1

2}, Nan Jiang^{1

2}, Linjin Fan^{1

2}, Xinxin Niu^{1

2}, Wenying Zhang^{1

2}, Mengmeng Huang^{1

2}, Li Gao^{1

2}, Kai Li¹, Yulong Gao^{1

2}, Changjun Liu¹, Hongyu Cui^{1

2}, Aijing Liu^{1

2}, Qing Pan¹, Yanping Zhang¹, Xiaomei Wang^{1

2

3}, Xiaole Qi^{1

2}

Affiliations

¹ State Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.
² OIE Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.
³ Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Disease and Zoonosis, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Abstract

Infectious bursal disease virus (IBDV) is a non-enveloped, bi-segmented double-stranded RNA virus and the causative agent of a poultry immunosuppressive disease known as infectious bursal disease (IBD). The novel variant IBDV (nVarIBDV) recently posed a great threat to the development of the poultry industry. In this study, we identified a novel segment-reassortant IBDV strain, IBDV-JS19-14701 (Genotype A2dB3). Phylogenic analysis showed that Segments A and B of IBDV-JS19-14701 were derived from emerging nVarIBDV (Genotype A2dB1) and long-prevalent HLJ0504-like strains (Genotype A3B3) in China, respectively. The pathogenicity of IBDV-JS19-14701 was further evaluated via animal experiments. IBDV-JS19-14701 exhibited a similar virulence to chickens with the nVarIBDV. The identification of this reassortment event is beneficial for understanding the epidemiology of nVarIBDV and will contribute to the efficient prevention and control of IBD.

Keywords: genome; infectious bursal disease virus; novel variant strain; pathogenicity; segment reassortment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Birnaviridae Infections / veterinary*
Birnaviridae Infections / virology
Chickens / virology
Genome, Viral*
Genotype*
Infectious bursal disease virus / classification
Infectious bursal disease virus / genetics*
Infectious bursal disease virus / pathogenicity*
Phylogeny
Poultry Diseases / virology
Reassortant Viruses / genetics*
Specific Pathogen-Free Organisms
Virulence / genetics