Prognostic Impact of Genetic Variants of MECP2 and TIRAP on Clinical Outcomes of Systemic Lupus Erythematosus with and without Nephritis

Safaa I Tayel; Nashwa M Muharram; Dina S Fotoh; Hany S Elbarbary; Huda I Abd-Elhafiz; Eman A El-Masry; Ahmed E Taha; Shimaa E Soliman

doi:10.3390/biom11091378

Prognostic Impact of Genetic Variants of MECP2 and TIRAP on Clinical Outcomes of Systemic Lupus Erythematosus with and without Nephritis

Biomolecules. 2021 Sep 18;11(9):1378. doi: 10.3390/biom11091378.

Authors

Safaa I Tayel^{1

2}, Nashwa M Muharram^{1

2}, Dina S Fotoh³, Hany S Elbarbary^{4

5}, Huda I Abd-Elhafiz⁶, Eman A El-Masry^{7

8}, Ahmed E Taha^{7

9}, Shimaa E Soliman^{1

10}

Affiliations

¹ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
² Medical Biochemistry Unit, College of Medicine, Al Baha University, Al Baha 65779, Saudi Arabia.
³ Physical Medicine, Rheumatology and Rehabilitation Department, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
⁴ Renal Unit, Department of Internal Medicine, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
⁵ Renal Unit, Department of Internal Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
⁶ Clinical Pharmacology Department, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
⁷ Microbiology and Immunology Unit, Department of Pathology, College of Medicine, Jouf University, Sakaka 72388, Saudi Arabia.
⁸ Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
⁹ Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
¹⁰ Medical Biochemistry Unit, Department of Pathology, College of Medicine, Qassim University, Buraydah 51452, Saudi Arabia.

Abstract

Systemic lupus erythematosus (SLE) is a chronic autoimmune illness with a growing prevalence in many populations. Few studies have examined genetic predisposition to SLE, so we aimed to examine the clinical impact of the genetic polymorphisms MECP2 rs2734647and TIRAP rs8177374 on the outcomes and therapeutic precision of SLE with and without nephritis. This study included 110 SLE patients-divided into 63 with lupus nephritis (LN), and 47 without nephritis-and 100 controls. Laboratory measurements including CRP, ESR, ACR, CBC, anti-ds-DNA, vitamin A, C3, and C4 were carried out, along with genotyping of MECP2 rs2734647and TIRAP rs8177374 by real-time PCR and sequencing. Treg %, vitamin A, C3, and C4 were lower, whereas Th17 % was higher, in patients vs. controls (p < 0.001). The T allele of MECP2 rs2734647 was higher in LN than in non-nephritis and control subjects. Moreover, the T allele of TIRAP rs8177374 was higher in LN than in non-nephritis and control subjects. The MECP2 and TIRAP genes could play a role in predisposition to SLE, and can also predict disease progress to nephritis, helping to personalize medicine.

Keywords: MECP; TIRAP; arthritis; nephritis; systemic lupus erythematosus.

MeSH terms

Adult
Blood Sedimentation
Female
Genetic Predisposition to Disease*
Genetic Variation*
Humans
Lupus Nephritis / genetics*
Membrane Glycoproteins / genetics*
Methyl-CpG-Binding Protein 2 / genetics*
Polymorphism, Single Nucleotide / genetics
Prognosis
Receptors, Interleukin-1 / genetics*
Treatment Outcome

Substances

MECP2 protein, human
Membrane Glycoproteins
Methyl-CpG-Binding Protein 2
Receptors, Interleukin-1
TIRAP protein, human