Plant resistance (R) proteins play a significant role in the detection of pathogen invasion. Accurately predicting plant R proteins is a key task in phytopathology. Most plant R protein predictors are dependent on traditional feature extraction methods. Recently, deep representation learning methods have been successfully applied in solving protein classification problems. Motivated by this, we propose a new computational approach, called prPred-DRLF, which uses deep representation learning feature models to encode the amino acids as numerical vectors. The results show that the fused features of bidirectional long short-term memory (BiLSTM) embedding and unified representation (UniRep) embedding have a better performance than other features for plant R protein identification using a light gradient boosting machine (LGBM) classifier. The model was evaluated using an independent test achieving an accuracy of 0.956, F1-score of 0.933, and area under the receiver operating characteristic (ROC) curve (AUC) of 0.997. Meanwhile, compared with the state-of-the-art prPred and HMMER method, prPred-DRLF shows an overall improvement in accuracy, F1-score, AUC, and recall. prPred-DRLF is a higher-performance plant R protein prediction tool based on two kinds of deep representation learning technologies and offers a user-friendly interface for inspecting possible plant R proteins. We hope that prPred-DRLF will become a useful tool for biological research. A user-friendly webserver for prPred-DRLF is freely accessible at http://lab.malab.cn/soft/prPred-DRLF. The Python script can be downloaded from https://github.com/Wangys-prog/prPred-DRLF.
Keywords: bidirectional long short-term memory; deep representation learning; light gradient boosting; plant R proteins; unified representation.
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