Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

Rajesh K Chaurasia; Kapil B Shirsath; Balvinder K Sapra

doi:10.1016/j.xpro.2021.100834

Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

STAR Protoc. 2021 Sep 15;2(4):100834. doi: 10.1016/j.xpro.2021.100834. eCollection 2021 Dec 17.

Authors

Rajesh K Chaurasia^{1

2}, Kapil B Shirsath¹, Balvinder K Sapra^{1

2}

Affiliations

¹ Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, Maharashtra 400094, India.
² Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra 400094, India.

Abstract

Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temperature, 4-35^oC) in whole blood samples in one step. FCS can be used for storing and transporting blood at ambient temperatures for up to 4 months, without altering the nuclear morphology and genomic integrity of WBCs.

Keywords: Clinical Protocol; Health Sciences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Platelets*
Erythrocytes
Fixatives
Humans
Leukocyte Count
Leukocytes*
Temperature

Substances

Fixatives