Quantitative proteome analysis reveals changes of membrane transport proteins in Sedum plumbizincicola under cadmium stress

Yue Zhu; Wenmin Qiu; Yuhong Li; Jinjuan Tan; Xiaojiao Han; Longhua Wu; Yugen Jiang; Zhiping Deng; Chao Wu; Renying Zhuo

doi:10.1016/j.chemosphere.2021.132302

Quantitative proteome analysis reveals changes of membrane transport proteins in Sedum plumbizincicola under cadmium stress

Chemosphere. 2022 Jan;287(Pt 3):132302. doi: 10.1016/j.chemosphere.2021.132302. Epub 2021 Sep 18.

Authors

Yue Zhu¹, Wenmin Qiu², Yuhong Li², Jinjuan Tan³, Xiaojiao Han², Longhua Wu⁴, Yugen Jiang⁵, Zhiping Deng⁶, Chao Wu⁷, Renying Zhuo⁸

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, PR China; Forestry Faculty, Nanjing Forestry University, Nanjing, 210037, PR China; Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, 311400, PR China.
² State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, PR China; Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, 311400, PR China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, PR China.
⁴ Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.
⁵ Agricultural Technology Extension Center of Fuyang District, Hangzhou, Zhejiang, 311400, PR China.
⁶ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, PR China. Electronic address: zhipingdeng@mail.zaas.ac.cn.
⁷ Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang, 310021, PR China. Electronic address: semporna@126.com.
⁸ State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, PR China; Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, 311400, PR China. Electronic address: zhuory@gmail.com.

PMID: 34563781
DOI: 10.1016/j.chemosphere.2021.132302

Abstract

Sedum plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins, in Cd detoxification of plants have received attention in recent years, but a comprehensive profiling of Cd-responsive membrane proteins from Cd hyperaccumulator plants is lacking. In this study, the membrane proteins of root, stem, and leaf tissues of S. plumbizincicola seedlings treated with Cd solution for 0, 1 or 4 days were analyzed by Tandem Mass Tag (TMT) labeling-based proteome quantification (Data are available via ProteomeXchange with identifier PXD025302). Total 3353 proteins with predicted transmembrane helices were identified and quantified in at least one tissue group. 1667 proteins were defined as DAPs (differentially abundant proteins) using fold change >1.5 with p-values <0.05. The number of DAPs involved in metabolism, transport protein, and signal transduction was significantly increased after exposure to Cd, suggesting that the synthesis and decomposition of organic compounds and the transport of ions were actively involved in the Cd tolerance process. The number of up-regulated transport proteins increased significantly from 1-day exposure to 4-day exposure, from 5 to 112, 16 to 42, 18 to 44, in root, stem, and leaf, respectively. Total 352 Cd-regulated transport proteins were identified, including ABC transporters, ion transport proteins, aquaporins, proton pumps, and organic transport proteins. Heterologous expression of SpABCB28, SpMTP5, SpNRAMP5, and SpHMA2 in yeast and subcellular localization showed the Cd-specific transport activity. The results will enhance our understanding of the molecular mechanism of Cd hypertolerance and hyperaccumulation in S. plumbizincicola and will be benefit for future genetic engineering in phytoremediation.

Keywords: Cadmium tolerance and accumulation; Integrative membrane protein; Sedum plumbizincicola; TMT labeling Based quantification proteome; Transporter.

MeSH terms

Biodegradation, Environmental
Cadmium / analysis
Membrane Transport Proteins
Proteome
Sedum* / metabolism
Soil Pollutants* / analysis

Substances

Membrane Transport Proteins
Proteome
Soil Pollutants
Cadmium