In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe

Philipp Doppler; Christoph Gasser; Ricarda Kriechbaum; Ardita Ferizi; Oliver Spadiut

doi:10.3390/bioengineering8090129

In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe

Bioengineering (Basel). 2021 Sep 21;8(9):129. doi: 10.3390/bioengineering8090129.

Authors

Philipp Doppler¹, Christoph Gasser², Ricarda Kriechbaum¹, Ardita Ferizi², Oliver Spadiut¹

Affiliations

¹ Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.
² usePAT GmbH, Schönbrunner Strasse 231/2.01, 1120 Vienna, Austria.

Abstract

Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophisticated process monitoring. Spectroscopic probes supported by ultrasound particle traps provide a powerful technology for in-line, nondestructive, and real-time process analytics in photobioreactors. This work shows the great potential of using ultrasound particle manipulation to improve spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra as a monitoring tool for PHB production processes in photobioreactors.

Keywords: PAT; PHB; Synechocystis sp. PCC 6714; polyhydroxyalkanoates; process monitoring; ultrasound particle manipulation.

Abstract

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