This study aimed at developing a cell-based encapsulation carrier for topical delivery of bioactives to the skin. The overall objectives were to evaluate affinity of the yeast-cell based carrier to bind to the skin surface following topical application and to quantify controlled release of curcumin as a model bioactive in ex-vivo skin models using a combination of imaging, modeling and analytical measurements. Both porcine skin tissue and clinically obtained human skin biopsies were studied. The results demonstrated that upon incubation with the ex-vivo skin tissues, the cell carriers rapidly bound to the skin surface following topical delivery and provided sustained release of encapsulated curcumin. The microcarrier binding and penetration of curcumin in the dermal compartment also showed to increase with incubation time. The average flux of curcumin in human skin biopsies Jp was 0.89 ± 0.02 μg/cm2/h. These results illustrated the potential of a novel cell-based carrier for high affinity binding to skin surface, efficient encapsulation of a model bioactive and controlled release from the cell carrier to the skin with enhanced permeation to the dermis section. Overall, this study demonstrated a new class of cost-effective carriers for improving delivery of bioactives to the skin and potentially other epithelial tissues.
Keywords: Confocal laser scanning microscopy; Curcumin; HPLC; Human skin; Transdermal dermal delivery; Yeast cell carriers.
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