Negative correlations between cultivable and active-yet-uncultivable pyrene degraders explain the postponed bioaugmentation

Bo Jiang; Yating Chen; Yi Xing; Luning Lian; Yaoxin Shen; Baogang Zhang; Han Zhang; Guangdong Sun; Junyi Li; Xinzi Wang; Dayi Zhang

doi:10.1016/j.jhazmat.2021.127189

Negative correlations between cultivable and active-yet-uncultivable pyrene degraders explain the postponed bioaugmentation

J Hazard Mater. 2022 Feb 5;423(Pt B):127189. doi: 10.1016/j.jhazmat.2021.127189. Epub 2021 Sep 12.

Authors

Bo Jiang¹, Yating Chen², Yi Xing², Luning Lian², Yaoxin Shen², Baogang Zhang³, Han Zhang³, Guangdong Sun⁴, Junyi Li⁵, Xinzi Wang⁴, Dayi Zhang⁶

Affiliations

¹ School of Energy and Environmental Engineering, University of Science & Technology Beijing, Beijing 100083, PR China; Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science & Technology Beijing, Beijing 100083, PR China; National Engineering Laboratory for Site Remediation Technologies, Beijing 100015, PR China.
² School of Energy and Environmental Engineering, University of Science & Technology Beijing, Beijing 100083, PR China; Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science & Technology Beijing, Beijing 100083, PR China.
³ School of Water Resources and Environment, MOE Key Lab Groundwater Circulation and Environment Evolution, China University of Geosciences, Beijing 100083, PR China.
⁴ School of Environment, Tsinghua University, Beijing 100084, PR China.
⁵ Department of Research and Development, Yiqing (Suzhou) Environmental Technology Co. Ltd, Suzhou 215163, PR China.
⁶ College of New Energy and Environment, Jilin University, Changchun 130021, PR China. Electronic address: zhangdayi@tsinghua.org.cn.

PMID: 34555764
DOI: 10.1016/j.jhazmat.2021.127189

Abstract

Bioaugmentation is an effective approach to remediate soils contaminated by polycyclic aromatic hydrocarbons (PAHs), but suffers from unsatisfactory performance in engineering practices, which is hypothetically explained by the complicated interactions between indigenous microbes and introduced degraders. This study isolated a cultivable pyrene degrader (Sphingomonas sp. YT1005) and an active pyrene degrading consortium (Gp16, Streptomyces, Pseudonocardia, Panacagrimonas, Methylotenera and Nitrospira) by magnetic-nanoparticle mediated isolation (MMI) from soils. Pyrene biodegradation was postponed in bioaugmentation with Sphingomonas sp. YT1005, whilst increased by 30.17% by the active pyrene degrading consortium. Pyrene dioxygenase encoding genes (nidA, nidA3 and PAH-RHDα-GP) were enriched in MMI isolates and positively correlated with pyrene degradation efficiency. Pyrene degradation by Sphingomonas sp. YT1005 only followed the phthalate pathway, whereas both phthalate and salicylate pathways were observed in the active pyrene degrading consortium. The results indicated that the uncultivable pyrene degraders were suitable for bioaugmentation, rather than cultivable Sphingomonas sp. YT1005. The negative correlations between Sphingomonas sp. YT1005 and the active-yet-uncultivable pyrene degraders were the underlying mechanisms of bioaugmentation postpone in engineering practices.

Keywords: Bioaugmentation; Degradation pathway; Magnetic nanoparticle-mediated isolation (MMI); Pyrene; Soils.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Polycyclic Aromatic Hydrocarbons* / analysis
Pyrenes
Soil Microbiology
Soil Pollutants* / analysis

Substances

Polycyclic Aromatic Hydrocarbons
Pyrenes
Soil Pollutants