Construction of a prognostic signature in Ewing's sarcoma: Based on metabolism-related genes

Zhaoyu Fu; Bo Yu; Mingxi Liu; Bo Wu; Yuanyuan Hou; Hongyu Wang; Yuting Jiang; Dong Zhu

doi:10.1016/j.tranon.2021.101225

Construction of a prognostic signature in Ewing's sarcoma: Based on metabolism-related genes

Transl Oncol. 2021 Dec;14(12):101225. doi: 10.1016/j.tranon.2021.101225. Epub 2021 Sep 21.

Authors

Zhaoyu Fu¹, Bo Yu², Mingxi Liu³, Bo Wu⁴, Yuanyuan Hou⁵, Hongyu Wang⁶, Yuting Jiang⁷, Dong Zhu⁸

Affiliations

¹ Department of Orthopaedics, the First Hospital of Jilin University, Street Xinmin 71, Changchun, China. Electronic address: fuzy19@mails.jlu.edu.cn.
² Clinical College, Jilin University, Street Xinmin 126, Changchun, China. Electronic address: yubo7018@jlu.edu.cn.
³ Department of Orthopaedics, the First Hospital of Jilin University, Street Xinmin 71, Changchun, China. Electronic address: liumingxi@jlu.edu.cn.
⁴ Department of Orthopaedics, the First Hospital of Jilin University, Street Xinmin 71, Changchun, China. Electronic address: wubo20@mails.jlu.edu.cn.
⁵ Clinical College, Jilin University, Street Xinmin 126, Changchun, China. Electronic address: houyy7018@mails.jlu.edu.cn.
⁶ Clinical College, Jilin University, Street Xinmin 126, Changchun, China. Electronic address: wanghy7018@mails.jlu.edu.cn.
⁷ Clinical College, Jilin University, Street Xinmin 126, Changchun, China. Electronic address: jiangyt7018@jlu.edu.cn.
⁸ Department of Orthopaedics, the First Hospital of Jilin University, Street Xinmin 71, Changchun, China. Electronic address: zhu_dong@mail.jlu.edu.cn.

Abstract

Objective: By combining the expression profiles of metabolism-related genes (MRGS) with clinical information, the expression quantities of MRGS and the influence on development and prognosis were systematically analyzed, so as to provide a theoretical basis for the clinical study on the prognosis of Ewing's sarcoma.

Methods: MRGs expression profiles of 64 patients with Ewing's sarcoma were obtained from GEO dataset. Univariate Cox regression analysis was used to identify metabolization-related differentially expressed genes (DEGs) related with prognosis in Ewing's sarcoma patients. Then, multivariate Cox analysis was used to calculate novel prognostic markers based on metabolism-related DEGs. Besides, We validate the model using ICGC datasets. Finally, the new prognostic index was verified on the basis of the prognostic models.

Results: Multivariate Cox regression analysis identified 74 metabolization-related DEGs, 25 of which were associated with Ewing's sarcoma patients' overall survival. Subsequently, we used 25 DEGs to construct metabolism-related prognostic signature for patients with Ewing's sarcoma. Based on the 18 DEGs regression coefficient, we propose the formula of each patient's risk score, and then divided the patients into high-risk group and low-risk group. The results indicated that the survival rate and survival time were higher in the low-risk group and lower in the high-risk group. Multivariate Cox analysis showed that risk score index was an independent prognostic factor for Ewing's sarcoma.

Conclusion: The experimental results suggest that the 18 metabolism-related DEGs marker may be effective in predicting the prognosis of Ewing's sarcoma to some extent, helping to individualize treatment of patients at different risks.

Keywords: Bioinformatics; Ewing's sarcoma; Metabolism-related genes; Prognosis.