Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food

Ximei Han; Ling Liu; Huiyu Gong; Linpin Luo; Yaru Han; Jiawen Fan; Chenfei Xu; Tianli Yue; Jianlong Wang; Wentao Zhang

doi:10.1016/j.foodchem.2021.131115

Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food

Food Chem. 2022 Mar 1:371:131115. doi: 10.1016/j.foodchem.2021.131115. Epub 2021 Sep 13.

Authors

Ximei Han¹, Ling Liu¹, Huiyu Gong¹, Linpin Luo¹, Yaru Han¹, Jiawen Fan¹, Chenfei Xu¹, Tianli Yue¹, Jianlong Wang¹, Wentao Zhang²

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China.
² College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China. Electronic address: zhangwt@nwsuaf.edu.cn.

PMID: 34555710
DOI: 10.1016/j.foodchem.2021.131115

Abstract

The total antioxidant capacity (TAC) has become increasingly vital for evaluating antioxidant food quality in the field of healthcare. Herein, a convenient and sensitive method for TAC assay was proposed based on the absorbance difference of reaction systems between various antioxidants existed in food and Dex-FeMnzyme/oxTMB. Under the optimum condition, the limit of detection (LOD) of the colorimetric sensor was 1.17 μM with the linear concentration range from 1 μM to 30 μM. The analysis results demonstrated the excellent feasibility of practical application in fruit and vegetable food, which offered a new avenue for the establishment of colorimetric biosensors.

Keywords: Antioxidant; Colorimetric sensor; Dex-FeMnzyme; Oxidase-like activity; Total antioxidant capacity (TAC).

MeSH terms

Antioxidants* / analysis
Colorimetry
Dextrans
Fruit* / chemistry
Oxidation-Reduction
Oxidoreductases
Vegetables

Substances

Antioxidants
Dextrans
Oxidoreductases