Immunization of chickens with Salmonella gallinarium ghosts expressing Salmonella Enteritidis NFliC-FimAC and CD40LC fusion antigen enhances cell-mediated immune responses and protects against wild-type challenges with both species

Amal Senevirathne; Chamith Hewawaduge; John Hwa Lee

doi:10.1016/j.dci.2021.104265

Immunization of chickens with Salmonella gallinarium ghosts expressing Salmonella Enteritidis ^NFliC-FimA^C and CD40L^C fusion antigen enhances cell-mediated immune responses and protects against wild-type challenges with both species

Dev Comp Immunol. 2022 Jan:126:104265. doi: 10.1016/j.dci.2021.104265. Epub 2021 Sep 24.

Authors

Amal Senevirathne¹, Chamith Hewawaduge¹, John Hwa Lee²

Affiliations

¹ College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 54596, Iksan, South Korea.
² College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 54596, Iksan, South Korea. Electronic address: johnhlee@jbnu.ac.kr.

PMID: 34555416
DOI: 10.1016/j.dci.2021.104265

Abstract

This study describes the construction and immunological characterization of a novel Salmonella gallinarium ghost vaccine to protect against S. gallinarium (SG) and S. Enteritidis (SE) serotypes. The SG ghost was designed to express N-terminus FliC (D0-D1 domain) and FimA retrieved from the SE genome, and the receptor-binding domain (RBD) of CD40L from the chicken as a single fusion construct. The construct was built in pJHL184, a phage lysis gene E-mediated ghost plasmid and the expression was confirmed by western blot resulting in an 85-kDa band. Chicken immunization was conducted by intramuscular route with SG ghost FliC-FimA-CD40L, vector control, or PBS alone in a prime-boost schedule. Antibody responses, cell-mediated immune responses (CMI), and cytokine induction was assessed in chicken demonstrating significantly high levels of IgY, CMI, cytokine responses in ghost immunized group delivering partial protection against SG wild type challenge and near complete protection against SE challenge wild type challenge.

Keywords: CD40L; FimA; FliC; Lysis gene E; Salmonella Enteritidis; Salmonella gallinarium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD40 Ligand
Chickens
Immunity, Cellular
Immunization / methods
Poultry Diseases* / prevention & control
Salmonella Infections, Animal* / prevention & control
Salmonella enteritidis / genetics

Substances

CD40 Ligand