Promoter analysis of the SPATULA (FvSPT) and SPIRAL (FvSPR) genes in the woodland diploid strawberry (Fragaria vesca L.)

Biol Futur. 2021 Sep;72(3):373-384. doi: 10.1007/s42977-021-00089-x. Epub 2021 Jun 9.

Abstract

The aim of this study was to identify transcription factor (TF) binding sites and cis-regulatory elements (CREs) on the promoters of FvSPR1-like2 (SPIRAL) and FvSPT (SPATULA) genes in the woodland diploid strawberry (Fragaria vesca L.). We identified: (1) MYB59, WRKY25 and WRKY8 TFs which play a role in ethylene signaling; (2) ARF family of TFs which play a role in ARF-mediated auxin signaling on the promoter of FvSPR1-like2 gene; (3) ARR family of TFs which play a role in cytokinin signaling; (4) ERF family of TFs which play a role in ethylene signaling on the promoter of FvSPT. This bioinformatic analysis of TFs and CREs may provide a better understanding of the function of genes involved in, and the mechanism underlying, non-climateric ripening during strawberry fruit maturation.

Keywords: Agroinfiltration; Cis-regulatory elements; SPATULA; SPIRAL; Transcription factors; Transient gene expression.

MeSH terms

  • Fragaria / drug effects*
  • Fragaria / genetics
  • Germ Cells, Plant / drug effects*
  • Transcription Factors / genetics

Substances

  • Transcription Factors