Comet assay study of the genotoxic effect of T-2 and HT-2 toxins in chicken hepatocytes

Rubina Tunde Szabó; Maria Kovács-Weber; Márta Erdélyi; Krisztián Balogh; Natasa Fazekas; Ákos Horváth; Miklós Mézes; Balázs Kovács

doi:10.1556/019.70.2019.37

Comet assay study of the genotoxic effect of T-2 and HT-2 toxins in chicken hepatocytes

Biol Futur. 2019 Dec;70(4):330-335. doi: 10.1556/019.70.2019.37. Epub 2019 Dec 1.

Authors

Rubina Tunde Szabó¹, Maria Kovács-Weber², Márta Erdélyi³, Krisztián Balogh^{3

4}, Natasa Fazekas¹, Ákos Horváth⁵, Miklós Mézes^{3

4}, Balázs Kovács⁵

Affiliations

¹ Institute of Animal Husbandry, Faculty of Agricultural and Environmental Sciences, Szent István University, Páter Károly str. 1, H-2100, Gödöllo, Hungary.
² Institute of Animal Husbandry, Faculty of Agricultural and Environmental Sciences, Szent István University, Páter Károly str. 1, H-2100, Gödöllo, Hungary. weber.maria@mkk.szie.hu.
³ Department of Nutrition, Faculty of Agricultural and Environmental Sciences, Szent István University, Páter Károly str. 1, H-2100, Gödöllo, Hungary.
⁴ MTA-KE-SZIE Mycotoxins in the Food Chain Research Groups, Kaposvár University, Guba Sándor str. 40, H7400, Kaposvár, Hungary.
⁵ Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, Pater Karoly str. 1, H-2100, Gödöllo, Hungary.

PMID: 34554539
DOI: 10.1556/019.70.2019.37

Abstract

Background and aims: The aim of this study was to verify that the comet assay can be used to investigate the DNA damaging effects of T-2 and HT-2 toxins in the liver of broiler chickens. The comet assay is a favorable genotoxic analysis because it is cheap, simple, and can be used in many organisms and different tissues.

Materials and methods: Male broiler chickens were fed with T-2/HT-2 toxins-contaminated diet for 14 days. The comet assay was successfully adapted to chicken liver cells, and the DNA damage was determined by a decrease in the comet parameter (DNA % in the tail) in the experimental groups.

Results: The method of evaluation was found to be critical because DNA damage could not be detected exactly using the CometScore software, due to inaccurate separation of head and comet. However, this problem can be solved by visual evaluation.

Conclusion: In the case of the visual evaluation, each toxin-treated group differed significantly from the control group, indicating that the assay can be useful for the assessment of primary DNA damage caused by T-2/HT-2 toxins.

Keywords: CometScore; T-2/HT-2 mycotoxin; poultry; toxicity; visual evaluation.