Implant associated infections (IAI) and poor osseointegration are the two major causes for titanium implant failure, leading to subsequent financial burden and physical sufferings. Therefore, advanced implants with excellent anti-infection and osseointegration performance are needed. In this work, mussel-inspired tannic acid (TA) mediated layer-by-layer (LbL) self-assembly was used for fabricating bonded polyethylene glycol (PEG) and 8DSS (8 repeating units of aspartate-serine-serine) coatings (Ti/8DSS/PEG) on the surface of titanium implants. The coating is designed to simultaneously reduce bacterial adhesion through the super-hydrophilic effect of PEG and promote osseointegration through the effective biomineralization of 8DSS. The obtained Ti/8DSS/PEG implant exhibits superior anti-biofouling capabilities (anti-protein adhesion and anti-bacterial adhesion against S. aureus and E. coli) and excellent biocompatibility. Meanwhile, the Ti/8DSS/PEG implant accelerates osteoblast differentiation and presents significantly better osteogenic ability than bare titanium implants in vivo. This mussel-inspired TA mediated LbL self-assembly method is expected to provide a multifunctional and robust platform for surface engineering in bone repair.