The aim of the present study was to evaluate whether the addition of red propolis extract (RPE), in different roughage: concentrate (R:C) ratios, influences the intake, digestibility, ruminal parameters, and serum biochemistry of sheep. We used eight Santa Inês sheep with an average body weight of 29.45 ± 1.58 kg, housed in metabolism cages for 60 days, and distributed in two simultaneous Latin square designs in a 2 × 2 factorial scheme. The factors consisted of two R:C ratios (70:30 and 30:70) with or without the addition of 15 mL/day of RPE. No interactions were found (P > 0.05) between R:C ratios and with or without RPE. Sheep fed 30:70 ratio showed higher (P < 0.05) intake and dry matter (DM) digestibility and non-fibrous carbohydrates and lower (P < 0.05) intake and neutral detergent fiber (NDF) digestibility. The addition of RPE did not influence (P > 0.05) the intake or nutrients digestibility, but the sheep that received RPE had a higher (P < 0.05) ruminal pH and longer (P < 0.05) time of rumination (min/kg DM) compared to the group without propolis. The ruminal ammonia concentration was higher for sheep fed 70:30 ratio, but the concentrations of total protein and albumin did not differ between R:C ratios. The addition of 15 mL of RPE does not influence the intake, digestibility, ingestive behavior, and rumen ammonical nitrogen of sheep. There is no association between the R:C ratio and the addition of 15 mL/day of RPE for sheep.
Keywords: Additive; Digestibility; Intake; Ruminal parameters; Serum biochemistry.
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.