Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota

FEMS Microbiol Lett. 2021 Oct 4;368(18):fnab128. doi: 10.1093/femsle/fnab128.

Abstract

Strain Marseille-P3519T isolated from the fecal flora of a 25-year-old healthy French woman was a Gram-positive anaerobic bacterium, non-motile and non-spore forming. The 16S rRNA gene sequence of Marseille-P3519 showed 97.73% of sequence similarity with Limosilactobacillus reuteri DSM 20016, the closest species, phylogenetically. Furthermore, the average nucleotide identity of strain Marseille-3519 with its closest related species was 75.8% that was very below the recommended threshold (>95-96%). Its genome had 2 237 367 bp with 45.42 mol% of G + C content. Major fatty acids were C16:0 (50.8%), C18:1n9 (18.0%), C18:2n6 (9.8%) and C19:1n9 (8.9%). It was catalase negative and fermented glycerol, glucose, fructose, D-maltose, lactose and mannose. These findings support that strain Marseille-P3519 ( = CSURP3519 = CECT 30110) is a new member of the genus Limosilactobacillus for which the name Limosilactobacillus caccae sp. nov., is proposed.

Keywords: Limosilactobacillus caccae; bacteria; genome; human gut; taxonogenomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids
  • Female
  • Gastrointestinal Microbiome*
  • Humans
  • Lactobacillaceae* / chemistry
  • Lactobacillaceae* / classification
  • Lactobacillaceae* / genetics
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S