Role of transient receptor potential channel 6 in the osteogenesis of periodontal ligament cells

Li Wang; Jing Mi; Bingjing Sun; Gang Yang; Shangfen Liu; Meihua Chen; Liming Yu; Jie Pan; Yuehua Liu

doi:10.1016/j.intimp.2021.108134

Role of transient receptor potential channel 6 in the osteogenesis of periodontal ligament cells

Int Immunopharmacol. 2021 Nov:100:108134. doi: 10.1016/j.intimp.2021.108134. Epub 2021 Sep 20.

Authors

Li Wang¹, Jing Mi², Bingjing Sun², Gang Yang², Shangfen Liu³, Meihua Chen⁴, Liming Yu², Jie Pan², Yuehua Liu⁵

Affiliations

¹ Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China; Dental Department, Shanghai 1st People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
² Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China.
³ Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China.
⁴ Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China; Department of Periodontology, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.
⁵ Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China. Electronic address: liuyuehua@fudan.edu.cn.

PMID: 34547679
DOI: 10.1016/j.intimp.2021.108134

Abstract

Transient receptor potential channel 6 (TRPC6) is a receptor-operated Ca²⁺ channel that plays an important role in Ca²⁺ influx in the majority of non-excitable cells and influences calcium signalling and cellular responses. Therefore, the purpose of the present study was to gain insight into the role of TRPC6 in the osteogenesis of periodontal ligament cells (PDLCs). By western blot and immunohistochemical staining, the protein level of TRPC6 was found to be increased in a time-dependent manner during osteoblastic differentiation of PDLCs. In addition, the TRPC6 inhibitor SKF96365 was used to block the function of TRPC6 and inhibit osteoblastic differentiation of PDLCs. The TRPC6 activator hyperforin dicyclohexylammonium salt (hyperforin DCHA) was used to activate TRPC6 and promote osteoblastic differentiation of PDLCs. In vivo, wild-type mice showed better bone regeneration than TRPC6^-/- mice, suggesting that TRPC6 has notable osteogenic induction properties and is important for bone defect repair. In conclusion, the current data demonstrated that TRPC6 plays a significant role in osteoblastic differentiation of PDLCs, suggesting that it may be a promising therapeutic target in osteogenesis.

Keywords: Osteogenesis; Periodontal Ligament Cells; TRPC6; TRPC6(−/−) mice.

MeSH terms

Adolescent
Animals
Cell Differentiation
Cells, Cultured
Child
Female
Humans
Imidazoles / pharmacology
Male
Mice
Mice, Knockout
Osteoblasts / drug effects
Osteoblasts / metabolism*
Osteoblasts / pathology
Osteogenesis*
Periodontal Ligament / drug effects
Periodontal Ligament / metabolism*
Periodontal Ligament / pathology
Signal Transduction
Skull / drug effects
Skull / metabolism*
Skull / pathology
TRPC6 Cation Channel / drug effects
TRPC6 Cation Channel / genetics
TRPC6 Cation Channel / metabolism*
Terpenes / pharmacology

Substances

Imidazoles
TRPC6 Cation Channel
TRPC6 protein, human
Terpenes
Trpc6 protein, mouse
hyperforin dicyclohexylammonium
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole