We detected 2 natural, asymptomatic Plasmodium inui monoinfections in humans in Malaysia by using nested PCR on concentrated high-volume blood samples. We found a P. inui-positive Anopheles cracens mosquito in the same site as the human infections. Investigators should use ultrasensitive detection methods to identify simian malaria parasite transmission in humans.
Keywords: Anopheles; Malaysia; Plasmodium inui; humans; malaria; monkey; parasites; vector; vector-borne infections; zoonoses.