Detection of Mycobacterium tuberculosis complex antibodies in free-ranged wild boar and wild macaques in selected districts in Selangor and reevaluation of tuberculosis serodetection in captive Asian elephants in Pahang, Peninsular Malaysia

J Vet Med Sci. 2021 Oct 31;83(11):1702-1707. doi: 10.1292/jvms.21-0144. Epub 2021 Sep 20.

Abstract

Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, affecting several domestic animals, wildlife species and humans. The preliminary investigation was aimed to detect antibody against MTBC among indigenous wildlife which are free-ranged wild boar, free-ranged wild macaques and captive Asian elephants in selected areas of Selangor and elephant conservation centre in Pahang, respectively. The results indicate that MTBC serodetection rate in wild boar was 16.7% (7.3-33.5 at 95% confidence interval (CI)) using an in-house ELISA bPPD IgG and 10% (3.5-25.6 at 95% CI) by DPP®VetTB assay, while the wild macaques and Asian elephant were seronegative. The univariate analysis indicates no statistically significant difference in risk factors for sex and age of wild boar but there was a significant positive correlation (P<0.05) between bovine TB in dairy cattle and wild boar seropositivity in the Sepang district.

Keywords: ELISA; Mycobacterium tuberculosis complex; antibody; wild boar; wildlife.

MeSH terms

  • Animals
  • Antibodies, Bacterial
  • Cattle
  • Cattle Diseases*
  • Elephants*
  • Macaca
  • Malaysia / epidemiology
  • Mycobacterium bovis*
  • Mycobacterium tuberculosis*
  • Seroepidemiologic Studies
  • Sus scrofa
  • Swine
  • Swine Diseases*
  • Tuberculosis* / diagnosis
  • Tuberculosis* / veterinary

Substances

  • Antibodies, Bacterial