Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)

Miguel Bernabé-Rubio; David C Gershlick; Miguel A Alonso

doi:10.21769/BioProtoc.2244

Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)

Bio Protoc. 2017 Apr 20;7(8):e2244. doi: 10.21769/BioProtoc.2244.

Authors

Miguel Bernabé-Rubio¹, David C Gershlick², Miguel A Alonso¹

Affiliations

¹ Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Madrid, Spain.
² Cell Biology and Neurobiology Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA.

Abstract

In polarised epithelial cells the midbody forms at the apical cell surface during cytokinesis. Once severed, the midbody is inherited by one of the daughter cells remaining tethered to the apical plasma membrane where it participates in non-cytokinetic processes, such as primary ciliogenesis. Here, we describe a novel method to physically remove the midbody remnant from cells and assess the possible effects caused by its loss (Bernabé- Rubio et al., 2016 ).

Keywords: Epithelial cells; Midbody remnant; Patch-clamp equipment; Primary cilium; Suction pressure.