Photometric Assays for Chloroplast Movement Responses to Blue Light

Halina Gabryś; Agnieszka Katarzyna Banaś; Paweł Hermanowicz; Weronika Krzeszowiec; Sebastian Leśniewski; Justyna Łabuz; Olga Sztatelman

doi:10.21769/BioProtoc.2310

Photometric Assays for Chloroplast Movement Responses to Blue Light

Bio Protoc. 2017 Jun 5;7(11):e2310. doi: 10.21769/BioProtoc.2310.

Authors

Halina Gabryś¹, Agnieszka Katarzyna Banaś¹, Paweł Hermanowicz¹, Weronika Krzeszowiec¹, Sebastian Leśniewski¹, Justyna Łabuz^{1

2}, Olga Sztatelman^{1

3}

Affiliations

¹ Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
² Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, Krakow, Poland.
³ Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, Warsaw, Poland.

Abstract

Assessment of chloroplast movements by measuring changes in leaf transmittance is discussed, with special reference to the conditions necessary for reliable estimation of blue light-activated chloroplast responses.

Keywords: Arabidopsis thaliana; Blue light; Chloroplast movements; Leaf transmittance; Photometric method; Phototropins.