Objective: To explore the mechanisms by which long non-coding RNA, (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) regulates cell proliferation, invasion and apoptosis through signaling axes in cutaneous squamous cell carcinoma (CSCC) cells.
Methods: A total of 60 CSCC samples and 15 normal skin tissue samples were collected. qRT-PCR was used to determine MALAT1 expression. After knockdown of MALAT1 expression in A431 cells, Transwell assay was performed to detect cell migration and invasion, CCK8 assay was used to detect cell proliferation, and Western blotting was used to detect EMT-related protein expression.
Results: Compared with the normal group, the MALAT1 positive expression rate was significantly higher in the low, moderate, and high differentiation groups (P < 0.05). The expression of MALATl in A431 cells in the siMALATl-1 and siMALATl-2 groups was lower than that in siNC group (P < 0.05). A431 cell proliferation, invasion and apoptosis at 24 h, 48 h and 72 h in the siMALATl-1 and siMALATl-2 groups were all lower and the apoptosis rate of A431 cells were all higher than that of the siNC group (P < 0.05). E-cadherin expression was higher while the expression of β-catenin, vimentin, and Bcl-2 was lower in the siMALATl-1 and siMALATl-2 groups than those of the siNC group (P < 0.05).
Conclusion: Down-regulation of lncRNA MALAT1 expression may promote apoptosis of CSCC cells and inhibit cell migration, invasion and proliferation by regulating the Wnt signaling pathway.
Keywords: Cutaneous squamous cell carcinoma; apoptosis; cell proliferation; invasion; long non-coding RNA.
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