Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay

Yang-Guang Ren; Mei-Chen Liu; Meng-Zhen Ji; Chen Chen; Hang-Zhan Hu; Zi-Xuan Wang; Pin-Qian Yu; Jia-Ming Shang; Qian-Wen Zhou; Ning-Ya Tao; Ya-Fei Guo; Yan-Jiao Lu; Zhi-Zeng Wang

doi:10.3892/etm.2021.10673

Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay

Exp Ther Med. 2021 Nov;22(5):1238. doi: 10.3892/etm.2021.10673. Epub 2021 Aug 31.

Authors

Affiliations

¹ Department of Breast and Thyroid Surgery, Huaihe Hospital of Henan University, Kaifeng, Henan 475004, P.R. China.
² Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, Henan 475004, P.R. China.
³ School of Medical Laboratory, Sanquan College of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

Abstract

The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic value. Therefore, in the present study, H-FABP was used as a biomarker in a double-antibody sandwich method and colloidal gold-based lateral flow immunoassay to develop a rapid detection kit for H-FABP with a processing time of only 5 min. The sensitivity of the kit in plasma and whole blood was 1 ng/ml and this method had good specificity, exhibiting no cross-reaction with cardiac troponin I, myoglobin or creatine kinase-Mb. The kits had good shelf life and stability, as they were able to be stored at 40˚C for 30 days. A total of 12 clinical samples were collected for detection and the coincidence rate with the ELISA method was up to 91.67%. Therefore, the present study provided a simple, rapid and economical early-detection in-home testing kit.

Keywords: acute myocardial infarction; blood; colloidal gold; heart-type fatty acid-binding protein; plasma; rapid detection.

Grants and funding

Funding: This work was supported by the Science and Technology Research Project of Kaifeng Science and Technology Bureau (grant no. 1903097), the Science and Technology Department of Henan Province (grant no. 182102310154) and the Key Scientific Research Project Plan of Colleges and Universities in Henan province (grant no. 19B320003).