Tristetraprolin Gene Single-Nucleotide Polymorphisms and mRNA Level in Patients With Rheumatoid Arthritis

Xiaoke Yang; Bo Chen; Mingyue Zhang; Shengqian Xu; Zongwen Shuai

doi:10.3389/fphar.2021.728015

Tristetraprolin Gene Single-Nucleotide Polymorphisms and mRNA Level in Patients With Rheumatoid Arthritis

Front Pharmacol. 2021 Sep 1:12:728015. doi: 10.3389/fphar.2021.728015. eCollection 2021.

Authors

Xiaoke Yang¹, Bo Chen², Mingyue Zhang³, Shengqian Xu¹, Zongwen Shuai¹

Affiliations

¹ Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
² Department of Nuclear Medicine, Chaohu Hospital Affiliated to Anhui Medical University, Hefei, China.
³ Department of Medical Record Room, Fuyang Hospital of Anhui Medical University, Fuyang, China.

Abstract

To observe and evaluate the correlation between single-nucleotide polymorphisms (SNPs) and messenger RNA (mRNA) level related to tristetraprolin (TTP) in Chinese rheumatoid arthritis (RA). TapMan SNP was used for genotyping analysis in 580 RA patients and 554 healthy people. Association between TTP gene polymorphisms (rs251864 and rs3746083) and RA was obtained. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technology was applied for the detection of TTP mRNA level in peripheral blood mononuclear cells (PBMCs) in 36 RA patients and 37 healthy people. We observed that the allele T of TTP rs3746083 increased RA susceptibility (p = 0.019). A significant difference was found under the dominant model of rs3746083 (p = 0.037). Further analysis showed the allele distribution of rs3746083 was nominally correlated with RF phenotype of RA patients (p = 0.045). Nevertheless, the association between TTP rs251864 and the incidence of RA was no statistically significant (p > 0.05). The TTP expression level in PBMCs of RA patients was significantly reduced (p < 0.001). In conclusion, the results of this experiment support that TTP may be involved in the pathogenesis of RA.

Keywords: TTP; expression; polymorphism; rheumatoid arthritis; tristetraprolin.