The Impact of β-1,4-Galactosyltransferase V on Microglial Function

Xiaoyu Wang; Naiqi Shi; Meiqi Hui; Hui Jin; Shumei Gao; Qiao Zhou; Li Zhang; Meijuan Yan; Hongmei Shen

doi:10.3389/fncel.2021.723308

The Impact of β-1,4-Galactosyltransferase V on Microglial Function

Front Cell Neurosci. 2021 Sep 3:15:723308. doi: 10.3389/fncel.2021.723308. eCollection 2021.

Authors

Xiaoyu Wang^{1

2}, Naiqi Shi³, Meiqi Hui¹, Hui Jin¹, Shumei Gao¹, Qiao Zhou¹, Li Zhang¹, Meijuan Yan¹, Hongmei Shen¹

Affiliations

¹ Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
² Department of Aoyang Cancer Institute, Affiliated Aoyang Hospital of Jiangsu University, Suzhou, China.
³ School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.

Abstract

β-1,4 Galactosyltransferase V (β-1,4-GalT V) belongs to the β-1,4 galactosyltransferase family, which modifies proteins and plays a vital role in biological function. Our previous study revealed that β-1,4-GalT V was expressed in the cortex and hippocampus and participated in the recovery of spatial learning and memory in rats with traumatic brain injury. However, the expression of β-1,4-GalT V in microglia, resident immune cells in the central nervous system, and its impact on microglia in resting and lipopolysaccharide-triggered activated stages are elusive. In this study, we clarified that β-1,4-GalT V expresses in microglia, and it regulates microglial migration, proliferation, and release of the inflammatory factors. We also observed that β-1,4-GalT V affects the expression level of tumor necrosis factor receptor (TNFR)2 instead of TNFR1. These results strongly support the fact that β-1,4-GalT V is involved in microglial function.

Keywords: 4-Galactosyltransferase V; interleukin-1β; lipopolysaccharide; microglia; migration; proliferation; tumor necrosis factor receptor; β-1.