Selenium Ameliorates S. aureus-Induced Inflammation in Bovine Mammary Epithelial Cells by Regulating ROS-Induced NLRP3 Inflammasome

Biol Trace Elem Res. 2022 Jul;200(7):3171-3175. doi: 10.1007/s12011-021-02924-7. Epub 2021 Sep 17.

Abstract

In this study, the regulation effects of selenium (Se) on the expression of pyrin domain-containing protein (NLRP) 3 inflammasome and reactive oxygen species (ROS) in bovine mammary epithelial cells (bMECs) infected by Staphylococcus aureus (S. aureus) were detected. bMECs were treated with 8 μmol/L Na2SeO3 for 12 h before infection with S. aureus for 2 h. Through flow cytometry, Western blot, and qRT-PCR analysis, the expression of ROS and NLRP3 imflammasome was detected. Results showed Se significantly reduced the ROS level in bMECs; at the same time, the expressions of NLRP3, ASC, caspase-1, Pro-IL-1β, and IL-1β were also decreased. In conclusion, Se inhibits S. aureus-induced inflammation by suppressing the activation of NLRP3 inflammasome and ROS in bMECs.

Keywords: NLRP3; ROS; S. aureus; Selenium.

MeSH terms

  • Animals
  • Cattle
  • Epithelial Cells / metabolism
  • Inflammasomes
  • Inflammation / drug therapy
  • Inflammation / metabolism
  • Interleukin-1beta / metabolism
  • Methicillin-Resistant Staphylococcus aureus* / metabolism
  • NLR Family, Pyrin Domain-Containing 3 Protein / metabolism
  • Reactive Oxygen Species / metabolism
  • Selenium* / metabolism
  • Selenium* / pharmacology
  • Signal Transduction
  • Staphylococcal Infections* / drug therapy
  • Staphylococcal Infections* / metabolism
  • Staphylococcus aureus / metabolism

Substances

  • Inflammasomes
  • Interleukin-1beta
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Reactive Oxygen Species
  • Selenium