Genome-wide analysis of the MYB-related transcription factor family and associated responses to abiotic stressors in Populus

Xiaoyu Yang; Ting Guo; Juan Li; Zhong Chen; Bin Guo; Xinmin An

doi:10.1016/j.ijbiomac.2021.09.042

Genome-wide analysis of the MYB-related transcription factor family and associated responses to abiotic stressors in Populus

Int J Biol Macromol. 2021 Nov 30:191:359-376. doi: 10.1016/j.ijbiomac.2021.09.042. Epub 2021 Sep 14.

Authors

Xiaoyu Yang¹, Ting Guo¹, Juan Li², Zhong Chen³, Bin Guo⁴, Xinmin An⁵

Affiliations

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
² Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; Key Laboratory of State Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China.
³ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China.
⁴ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; Shanxi Academy of Forest Sciences, Taiyuan, Shanxi 030012, China.
⁵ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: anxinmin@bjfu.edu.cn.

PMID: 34534587
DOI: 10.1016/j.ijbiomac.2021.09.042

Abstract

MYB proteins are one of the most abundant transcription factor families in the plant kingdom. Evidence has increasingly revealed that MYB-related proteins function in diverse plant biological processes. However, little is known about the genome-wide characterization and functions of MYB-related proteins in Populus, an important model and commercial tree species. In this study, 152 PtrMYBRs were identified and unevenly located on 19 Populus chromosomes. A phylogenetic analysis divided them into six major subgroups, supported by conserved gene organization, consensus motifs, and protein domain architecture. Promoter assessment and gene ontology classification results indicated that the MYB-related family is likely involved in plant development and responses to various environmental stressors. The Populus MYB-related family members showed various expression patterns in different tissues and stress conditions, implying their crucial roles in the development and stress responses in Populus. Co-expression analyses revealed that Populus MYB-related genes might participate in the regulation of antioxidant defense system and various signaling pathways in response to stress. The three-dimensional structures of different subgroup of Populus MYB-related proteins further provided functional information at the proteomic level. These findings provide valuable information for a prospective functional dissection of MYB-related proteins and genetic improvement of Populus.

Keywords: Classification; Expression pattern; MYB-related proteins; Phylogenetic analysis; Populus.

MeSH terms

Gene Expression Regulation, Plant
Plant Proteins / genetics*
Plant Proteins / metabolism
Populus / genetics*
Populus / metabolism
Stress, Physiological
Transcription Factors / genetics*
Transcription Factors / metabolism

Substances

Plant Proteins
Transcription Factors