The Vibrio parahaemolyticus subunit toxin PirBvp recognizes glycoproteins on the epithelium of the Penaeus vannamei hepatopancreas

Abstract

Vibrio parahaemolyticus toxin PirAB^vp is the major virulence factor exotoxin that contributes to the disruption of the hepatopancreatic epithelium in acute hepatopancreatic necrosis disease in shrimp. The PirB^vp subunit is a lectin that recognizes amino sugars; however, its potential role in recognition of the hepatopancreas has not been identified. In the present work, we identified the cellular receptor for PirB^vp in the shrimp hepatopancreas. A ligand blot assay of hepatopancreas lysate showed that the PirB^vp subunit recognizes two glycoprotein bands of 60 and 70 kDa (Gc60 and Gc70). The hepatopancreas lysate was fractionated by anion-exchange chromatography, and the three main fractions obtained contained the recognized Gc60 and Gc70 protein bands. LC-MS/MS indicated that beta-hexosaminidases subunit beta and mucin-like 5 AC corresponded to the 60 and 70 kDa bands, respectively, which seem to be expressed in the epithelial cells of the hepatopancreas. Endoglycosidase treatment of hepatopancreas lysate with the O-glycosidase from Enterococcus faecalis, inhibits the binding of PirB^vp. Altogether, these results suggest the relevance of the interaction of PirB^vp with the hepatopancreas in the pathogenesis of acute hepatopancreatic necrosis disease in shrimp.

Keywords: AHPND; Lectin; Mucin-like receptor; PirB(vp) toxin; beta-hexosaminidases subunit beta.