Manipulation of TAD reorganization by chemical-dependent genome linking

STAR Protoc. 2021 Sep 8;2(3):100799. doi: 10.1016/j.xpro.2021.100799. eCollection 2021 Sep 17.

Abstract

Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions. The advantages are that the manipulation does not change the genome and is reversible by withdrawing ABA. The major challenge is how to select linking loci for efficient TAD reorganization. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

Keywords: Biotechnology and bioengineering; CRISPR; Developmental biology; Genomics; MolecularBiology; Stem Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Animals
  • Cell Differentiation / genetics*
  • Cell Line
  • Cytological Techniques / methods*
  • Genome / drug effects
  • Genome / genetics
  • Genome Components* / drug effects
  • Genome Components* / genetics
  • Genomics / methods*
  • Humans
  • Mice

Substances

  • Abscisic Acid