Effects of in ovo Injection of Astragalus Polysaccharide on the Intestinal Development and Mucosal Immunity in Broiler Chickens

Shu-Bao Yang; Yan-Jun Qin; Xin Ma; Wei-Min Luan; Peng Sun; An-Qi Ju; Ao-Yi Duan; Ying-Nan Zhang; Dong-Hai Zhao

doi:10.3389/fvets.2021.738816

Effects of in ovo Injection of Astragalus Polysaccharide on the Intestinal Development and Mucosal Immunity in Broiler Chickens

Front Vet Sci. 2021 Aug 30:8:738816. doi: 10.3389/fvets.2021.738816. eCollection 2021.

Authors

Shu-Bao Yang^{1

2}, Yan-Jun Qin², Xin Ma², Wei-Min Luan², Peng Sun², An-Qi Ju², Ao-Yi Duan², Ying-Nan Zhang^{3

4}, Dong-Hai Zhao¹

Affiliations

¹ Basic Medical College, Jilin Medical University, Jilin, China.
² College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
³ School of Public Health, Jilin Medical University, Jilin, China.
⁴ College of Life Science, Changchun Sci-Tech University, Changchun, China.

Abstract

The purpose of this study was to examine the effects of in ovo injection of Astragalus polysaccharide (APS) on hatchability, body weight (BW), intestinal histomorphology, the number of IgA⁺ cells and sIgA content in intestine, and the expression of intestinal immune-related genes in broiler chickens. On day 18 of the incubation, a total of 960 live embryo eggs were weighed and randomly divided into 4 treatment groups: a control group and three APS groups. The eggs in the control group were injected with 0.5 mL physiological saline. The eggs in the APS groups were injected with 3 different amounts of APS in 0.5 mL physiological saline: 1 mg (APS_L), 2 mg (APS_M) and 4 mg (APS_H). The solution was injected into the amnion of each egg. The results showed that in ovo injection of APS did not affect the hatchability but increased the body weight of the 14 d and 21 d chickens, with a significant increase observed in the APS_M group (P < 0.05). At most time points, the villus height (VH) was increased (P < 0.05) and the crypt depth (CD) was decreased (P < 0.05) in the small intestine of the broilers, with higher VH/CD ratios in the APS_L and APS_M groups compared with the control group. The number of IgA⁺ cells in the mucosa and the secretory immunoglobulin A (sIgA) levels in the intestinal washings were higher in the APS_M and APS_H groups than in the APS_L and control groups. The gene expression levels of interleukin (IL)-2, interleukin (IL)-4, interferon gamma (IFN-γ), and Toll-like receptor (TLR)-4 were significantly enhanced by APS stimulation at most time points (P < 0.05). These results indicated that in ovo injection of APS has the potential of promoting intestinal development and enhancing intestinal mucosal immunity of broiler chickens in the early stage after hatching.

Keywords: Astragalus polysaccharide; broilers; in ovo injection; intestinal development; intestinal mucosal immunity.