Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

Natasha K Khatwani; Kelly J Andrews; Asha B Pillai

doi:10.1007/978-1-0716-1775-5_13

Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

Methods Mol Biol. 2021:2388:139-148. doi: 10.1007/978-1-0716-1775-5_13.

Authors

Natasha K Khatwani^#^{1

2

3}, Kelly J Andrews^#^{1

4

5}, Asha B Pillai^{6

7

8

9}

Affiliations

¹ Department of Pediatrics, University of Miami Miller School of Medicine, Miami, FL, USA.
² Sheila and David Fuente Program in Cancer Biology, University of Miami Miller School of Medicine, Miami, FL, USA.
³ Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.
⁴ Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, USA.
⁵ Beckman Coulter, Miami, FL, USA.
⁶ Department of Pediatrics, University of Miami Miller School of Medicine, Miami, FL, USA. asha.pillai@med.miami.edu.
⁷ Sheila and David Fuente Program in Cancer Biology, University of Miami Miller School of Medicine, Miami, FL, USA. asha.pillai@med.miami.edu.
⁸ Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, USA. asha.pillai@med.miami.edu.
⁹ Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA. asha.pillai@med.miami.edu.

^# Contributed equally.

Abstract

iNKT cells, classified as innate lymphocytes with invariant TCRs, have been highlighted as a putative, "off-the-shelf" cellular immunotherapeutic strategy for the treatment of malignant and nonmalignant diseases. However, their paucity in human blood limits their immunotherapeutic applications. Herein we describe a rigorously optimized 21-day ex vivo expansion method to achieve log-fold increases in immunotherapeutic human iNKT cells.

Keywords: Adoptive cellular therapy; Bone marrow transplantation; Cancer immunotherapy; Cytotoxicity; Hematopoietic stem cell transplantation; Immunotherapy; Leukemia; NKT cells; Transplantation; iNKT cells.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Humans
Immunotherapy
Natural Killer T-Cells*
Receptors, Antigen, T-Cell
Th2 Cells

Substances

Receptors, Antigen, T-Cell

Abstract

Publication types

MeSH terms

Substances

Grants and funding