Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

Wenjing He; Dongmei Ye; Yifang Gao

doi:10.1007/978-1-0716-1775-5_6

Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

Methods Mol Biol. 2021:2388:71-77. doi: 10.1007/978-1-0716-1775-5_6.

Authors

Wenjing He^{1

2

3}, Dongmei Ye^{1

2

3}, Yifang Gao^{4

5

6}

Affiliations

¹ Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P. R. China.
³ Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
⁴ Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. gaoyf26@sysu.edu.cn.
⁵ Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P. R. China. gaoyf26@sysu.edu.cn.
⁶ Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. gaoyf26@sysu.edu.cn.

PMID: 34524662
DOI: 10.1007/978-1-0716-1775-5_6

Abstract

It is widely accepted that iNKT cells are abundant in the liver and play a role in various liver disorders. In here, we describe an optimized protocol in identifying and isolating invariant natural killer T (iNKT) cells by magnetic beads to further use in functional assays.

Keywords: Flow cytometry; Proliferation; iNKT.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flow Cytometry
Humans
Liver
Natural Killer T-Cells*