Morphological, Biochemical, and Proteomic Studies Revealed Impact of Fe and P Crosstalk on Root Development in Phaseolus vulgaris L

Abstract

Mineral stress is one of the major abiotic stresses faced by crop plants. The present study was undertaken to investigate the impact of mineral stress (iron (Fe) and phosphorus (P)) on various morphological and biochemical responses of the shoot and root tissues and root architecture of common bean (Phaseolus vulgaris L.). This study also leads us to the identification of P stress responsive proteins. The study was conducted under in vitro conditions, in which seeds of Shalimar French Bean-1 (SFB-1) variety were cultured on four different MGRL medium (control (P₁Fe₁), iron deficient (P₁Fe₀), phosphorus deficient (P₀Fe₁), and phosphorus and iron deficient (P₀Fe₀)). Chlorophyll content of leaves, Fe/P content of root tissues, total sugars, proline, length, and weight of shoot and root tissues were assessed and compared within and between the treatments. The analyzed data revealed significant difference between control and other three treatments. Chlorophyll content of shoots was found significantly decreased under mineral stress treatments P₀Fe₁, P₁Fe₀, and P₀Fe₀ than control. Length and weight of shoot and root were also observed significantly decreased under P₀Fe₁, P₁Fe₀, and P₀Fe₀ as compared to control. Total sugar was significantly higher in P₀Fe₁ of roots in comparison to control. Proline content was significantly higher in both tissues of shoots and roots of plants grown under P₁Fe₀, P₀Fe₁, and P₀Fe₀ than control condition. Furthermore, we unexpectedly observed the recovery of roots (mainly primary roots) under P₀Fe₀ as compared to P₁Fe₀ and P₀Fe₁^. Interestingly higher concentration of Fe was also observed in P₀Fe₁ compared to other treatments and also higher concentration of P was observed in P₁Fe₁. These findings suggested that there is a crosstalk between Fe and P and also revealed that there is a disruption in the ability of PR (primary root) to sense local P deficiency in the absence of Fe. Furthermore, proteomics analysis (SDS-PAGE followed by MALDI MS) helped in identification of defensive proteins in P stress condition compared to control.

Keywords: Common bean; Crosstalk; In vitro; Iron; Mineral stress; Phosphorus; Proteomics; Root architecture.