Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

Chem Commun (Camb). 2021 Oct 5;57(79):10222-10225. doi: 10.1039/d1cc04066a.

Abstract

We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries.

MeSH terms

  • Antigens, Viral / chemistry*
  • Aptamers, Nucleotide / chemistry*
  • Biological Assay
  • COVID-19 / diagnosis*
  • COVID-19 Testing / methods*
  • Carbocyanines / chemistry
  • Coronavirus Nucleocapsid Proteins / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Phosphoproteins / chemistry
  • SARS-CoV-2*

Substances

  • Antigens, Viral
  • Aptamers, Nucleotide
  • Carbocyanines
  • Coronavirus Nucleocapsid Proteins
  • Fluorescent Dyes
  • Phosphoproteins
  • cyanine dye 3
  • nucleocapsid phosphoprotein, SARS-CoV-2