This study investigated the influence of temperature on the hydrolysis and decomposition of waste activated sludge (WAS) during the enhanced pretreatment system with lysozyme and rhamnolipid (Ly + RL). Results showed that temperature increasing from 15℃ to 55℃ could obviously enhance the release of soluble organic matters and WAS decomposition degree within the Ly + RL pretreatment system. Compared to the sum of sole Ly and sole RL pretreatment, Ly + RL combined pretreatment system at 45℃ presented best synergistic hydrolysis performance. The decomposition degree of bacteria and archaea reached 47.6% and 88.1%, respectively. Meanwhile, increasing temperature could recede the diversity of microbial community in the system. Gammaproteobacteria, with the relative abundance of 90.7%, occupied the absolute dominant position at 45℃. Furthermore, with the rise of temperature, more volatile fatty acids were harvested after anaerobic fermentation.
Keywords: Decomposition degree; Lysozyme; Rhamnolipid; Temperature; Waste activated sludge.
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