Stability and nutrients removal performance of a Phanerochaete chrysosporium-based aerobic granular sludge process by step-feeding and multi A/O conditions

Lihui Cui; Hao Shen; Pengfei Kang; Xiaoying Guo; Haisong Li; Yan Wang; Junfeng Wan; Christophe Dagot

doi:10.1016/j.biortech.2021.125839

Stability and nutrients removal performance of a Phanerochaete chrysosporium-based aerobic granular sludge process by step-feeding and multi A/O conditions

Bioresour Technol. 2021 Dec:341:125839. doi: 10.1016/j.biortech.2021.125839. Epub 2021 Sep 6.

Authors

Lihui Cui¹, Hao Shen¹, Pengfei Kang¹, Xiaoying Guo², Haisong Li², Yan Wang², Junfeng Wan³, Christophe Dagot⁴

Affiliations

¹ School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, PR China.
² School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, PR China; Henan International Joint Laboratory of Environment and Resources, Zhengzhou University, Zhengzhou 450001, PR China.
³ School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, PR China; GRESE EA 4330, Université de Limoges, 123 avenue Albert Thomas, F-87060 Limoges Cedex, France. Electronic address: wanjunfeng@zzu.edu.cn.
⁴ GRESE EA 4330, Université de Limoges, 123 avenue Albert Thomas, F-87060 Limoges Cedex, France; INSERM, U1092, Limoges, France.

PMID: 34523562
DOI: 10.1016/j.biortech.2021.125839

Abstract

A Phanerochaete chrysosporium-based aerobic granular sludge (PC-AGS) was developed by inoculating fungal mycelial pellets into a lab-scale aerobic granular sequencing batch reactor (AGSBR). A strategy using step-anaerobic feeding coupled with multi A/O conditions was adopted. The results showed that the removal efficiencies for total phosphorus (TP) and total inorganic nitrogen (TIN) were 94.56 ± 2.92% and 75.20 ± 7.74%, respectively, under relatively low aeration time. Compared with original AGS, the content of extracellular proteins for PC-AGS obviously increased from 18.61 to 41.44 mg/g MLSS by the end of phase I. Moreover, the mature granules had a larger size and better stability during the 100 days operation. Furthermore, the analysis of microbial diversity detected many key functional groups in PC-AGS granules that were beneficial to nutrients removal. This work demonstrated that the addition of fungal pellets not only enhanced the removal performance, but also improved the stability of the AGS system.

Keywords: Aerobic granular sludge; Mycelium pellets; Nutrients removal; Stability.

MeSH terms

Aerobiosis
Bioreactors
Nitrogen
Nutrients
Phanerochaete*
Phosphorus
Sewage*
Waste Disposal, Fluid

Substances

Sewage
Phosphorus
Nitrogen