Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation

Yanan Wang; Yingyan Zhou; Dongtang Zhang; Xiayan Wang; Shaorong Liu

doi:10.1016/j.heliyon.2021.e07904

Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation

Heliyon. 2021 Sep 7;7(9):e07904. doi: 10.1016/j.heliyon.2021.e07904. eCollection 2021 Sep.

Authors

Yanan Wang¹, Yingyan Zhou¹, Dongtang Zhang¹, Xiayan Wang¹, Shaorong Liu²

Affiliations

¹ Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, PR China.
² Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA.

Abstract

Hydrodynamic chromatography (HDC) is a technique originally developed for separating particles. We have recently extended it to DNA fragment sizing and quantitation. In this review, we focus on this extension. After we briefly introduce the history of HDC, we present the evolution of open tubular HDC for DNA fragment sizing. We cover both the theoretical aspect and the experimental implementation of this technique. We describe various approaches to execute the separation, discuss its representative applications and provide a future perspective of this technique in the conclusion section of this review.

Keywords: DNA fragment Quantitation; DNA fragment Sizing; Free solution separation; Hydrodynamic chromatography; Open tubular column.

Publication types

Review