New contributions to Diatrypaceae from karst areas in China

Sihan Long; Lili Liu; Yinhui Pi; Youpeng Wu; Yan Lin; Xu Zhang; Qingde Long; Yingqian Kang; Jichuan Kang; Nalin N Wijayawardene; Feng Wang; Xiangchun Shen; Qirui Li

doi:10.3897/mycokeys.83.68926

New contributions to Diatrypaceae from karst areas in China

MycoKeys. 2021 Aug 20:83:1-37. doi: 10.3897/mycokeys.83.68926. eCollection 2021.

Authors

Sihan Long^{1

2}, Lili Liu³, Yinhui Pi¹, Youpeng Wu¹, Yan Lin¹, Xu Zhang¹, Qingde Long¹, Yingqian Kang⁴, Jichuan Kang⁵, Nalin N Wijayawardene^{1

6}, Feng Wang⁷, Xiangchun Shen^{1

2}, Qirui Li^{1

2}

Affiliations

¹ State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China.
² The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources), School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
³ Immune Cells and Antibody Engineering Research Center of Guizhou Province/ Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang 550004, China.
⁴ Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou and Guizhou Talent Base for Microbiology and Human Health, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
⁵ Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, Guizhou 550025, China.
⁶ Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China.
⁷ Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science, Guiyang, Guizhou, 550000, China.

Abstract

In this study, fungal specimens of the family Diatrypaceae were collected from karst areas in Guizhou, Hainan and Yunnan Provinces, China. Morpho-molecular analyses confirmed that these new collections comprise a new genus Pseudodiatrype, three new species (Diatrypelancangensis, Diatrypellapseudooregonensis and Eutypacerasi), a new combination (Diatrypellaoregonensis), two new records (Allodiatrypethailandica and Diatrypellavulgaris) from China and two other known species (Neoeutypellabaoshanensis and Paraeutypellacitricola). The new taxa are introduced, based on multi-gene phylogenetic analyses (ITS, β-tubulin), as well as morphological analyses. The new genus Pseudodiatrype is characterised by its wart-like stromata with 5-20 ascomata immersed in one stroma and the endostroma composed of thin black outer and inner layers of large white cells with thin, powdery, yellowish cells. These characteristics separate this genus from two similar genera Allodiatrype and Diatrype. Based on morphological as well as phylogenetic analyses, Diatrypelancangensis is introduced as a new species of Diatrype. The stromata of Diatrypelancangensis are similar to those of D.subundulata and D.undulate, but the ascospores are larger. Based on phylogenetic analyses, Diatrypeoregonensis is transferred to the genus Diatrypella as Diatrypellaoregonensis while Diatrypellapseudooregonensis is introduced as a new species of Diatrypella with 8 spores in an ascus. In addition, multi-gene phylogenetic analyses show that Eutypacerasi is closely related to E.lata, but the ascomata and asci of Eutypacerasi are smaller. The polyphyletic nature of some genera of Diatrypaceae has led to confusion in the classification of the family, thus we discuss whether the number of ascospores per asci can still be used as a basis for classification.

Keywords: Xylariales; Five novel taxa; phylogeny; systematics; taxonomy.

Sihan Long, Lili Liu, Yinhui Pi, Youpeng Wu, Yan Lin, Xu Zhang, Qingde Long, Yingqian Kang, Jichuan Kang, Nalin N. Wijayawardene, Feng Wang, Xiangchun Shen, Qirui Li.