A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit

Irene Chiesa; Roberto Di Gesù; Kalon J Overholt; Riccardo Gottardi

doi:10.1007/978-1-0716-1693-2_16

A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit

Methods Mol Biol. 2022:2373:267-281. doi: 10.1007/978-1-0716-1693-2_16.

Authors

Irene Chiesa^{1

2

3

4}, Roberto Di Gesù^{3

4

5}, Kalon J Overholt^{4

6

7

8}, Riccardo Gottardi^{9

10

11}

Affiliations

¹ Research Center 'E. Piaggio', University of Pisa, Pisa, Italy.
² Department of Information Engineering, University of Pisa, Pisa, Italy.
³ Department of Pediatrics, Division of Pulmonary Medicine, The Children's Hospital of Philadelphia and The University of Pennsylvania, Philadelphia, PA, USA.
⁴ Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
⁵ Fondazione Ri.MED, Palermo, Italy.
⁶ Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
⁷ Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
⁸ Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
⁹ Department of Pediatrics, Division of Pulmonary Medicine, The Children's Hospital of Philadelphia and The University of Pennsylvania, Philadelphia, PA, USA. gottardir@email.chop.edu.
¹⁰ Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA. gottardir@email.chop.edu.
¹¹ Fondazione Ri.MED, Palermo, Italy. gottardir@email.chop.edu.

PMID: 34520018
DOI: 10.1007/978-1-0716-1693-2_16

Abstract

Interface tissues are functionally graded tissues characterized by a complex layered structure, which therefore present a great challenge to be reproduced and cultured in vitro. Here, we describe the design and operation of a 3D printed dual-chamber bioreactor as a culturing system for biphasic native or engineered osteochondral tissues. The bioreactor is designed to potentially accommodate a variety of interface tissues and enables the precise study of tissue crosstalk by creating two separate microenvironments while maintaining the tissue compartments in direct contact.

Keywords: Bioreactor; Biphasic constructs; Crosstalk; Interface tissue engineering; Microphysiological system; Osteochondral junction; Paracrine signaling.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors
Cartilage
Tissue Engineering*
Tissue Scaffolds

Grants and funding

U18 TR000532/TR/NCATS NIH HHS/United States