Miniaturized Vascularized Bleeding Model of Hemostasis

Elaissa Trybus Hardy; Yumiko Sakurai; Wilbur A Lam

doi:10.1007/978-1-0716-1693-2_10

Miniaturized Vascularized Bleeding Model of Hemostasis

Methods Mol Biol. 2022:2373:159-175. doi: 10.1007/978-1-0716-1693-2_10.

Authors

Elaissa Trybus Hardy^{1

2

3}, Yumiko Sakurai^{1

2}, Wilbur A Lam^{4

5

6}

Affiliations

¹ Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
² Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA.
³ Aflac Cancer and Blood Disorders Center of Children's Healthcare of Atlanta, Atlanta, GA, USA.
⁴ Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. wilbur.lam@emory.edu.
⁵ Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA. wilbur.lam@emory.edu.
⁶ Aflac Cancer and Blood Disorders Center of Children's Healthcare of Atlanta, Atlanta, GA, USA. wilbur.lam@emory.edu.

PMID: 34520012
DOI: 10.1007/978-1-0716-1693-2_10

Abstract

This chapter describes the development of a poly(dimethylsiloxane)-based microfluidic platform that is able to holistically assess and visualize the entire hemostatic process in vitro. The microfluidic platform includes (1) integration of intact endothelium, (2) physiological flow conditions, (3) controlled mechanical injury to study global hemostatic potential of a patient's whole blood samples in a microvascular model and for dissecting pathophysiologic mechanisms of diseases.

Keywords: Endothelium; Hemostasis; Injury model; Microfluidics; Microvasculature.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Hemorrhage
Hemostasis*
Hemostatics
Humans
Microfluidics

Substances

Hemostatics

Grants and funding

R35 HL145000/HL/NHLBI NIH HHS/United States