A dual lock-and-key two photon fluorescence probe in response to hydrogen peroxide and viscosity: Application in cellular imaging and inflammation therapy

Tianyu Liang; Dongliang Zhang; Wei Hu; Chenyang Tian; Lingyu Zeng; Tianhong Wu; Dongqing Lei; Taotao Qiang; Xuekang Yang; Xiaolong Sun

doi:10.1016/j.talanta.2021.122719

A dual lock-and-key two photon fluorescence probe in response to hydrogen peroxide and viscosity: Application in cellular imaging and inflammation therapy

Talanta. 2021 Dec 1:235:122719. doi: 10.1016/j.talanta.2021.122719. Epub 2021 Jul 15.

Authors

Tianyu Liang¹, Dongliang Zhang², Wei Hu³, Chenyang Tian², Lingyu Zeng⁴, Tianhong Wu⁵, Dongqing Lei⁵, Taotao Qiang⁶, Xuekang Yang⁷, Xiaolong Sun⁸

Affiliations

¹ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China; College of Bioresources and Materials Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China.
² Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi 'an, 710032, China.
³ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China; College of Bioresources and Materials Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China. Electronic address: 4574@sust.edu.cn.
⁴ Department of Chemistry, The University of Texas at Austin, Texas, 78712, United States.
⁵ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
⁶ College of Bioresources and Materials Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China.
⁷ Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi 'an, 710032, China. Electronic address: yangxuekangburns@163.com.
⁸ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China. Electronic address: x.l.sun86@xjtu.edu.cn.

PMID: 34517587
DOI: 10.1016/j.talanta.2021.122719

Abstract

Here, a dual lock-and-key fluorescence probe was developed for visualizing the inflammatory process in myocardial H9C2 cells. The probe possessed two-photon properties, viscosity sensitivity, and hydrogen peroxide (H₂O₂) responsiveness. A thiocarbamate spacer between fluorophore and H₂O₂ responsive unit enabled the release of carbonyl sulfide (COS). This rapidly converts to the anti-inflammatory hydrogen sulfide (H₂S) by the ubiquitous enzyme carbon anhydrase. The probe displayed a dual response towards hydrogen peroxide and viscosity in vitro. No obvious fluorescence changes were observed towards either hydrogen peroxide or viscosity alone. In cellular experiments, the probe demonstrated good biocompatibility, low toxicity, and was shown responses towards exogenous and endogenous hydrogen peroxide under viscosity conditions. LPS induced cell inflammation showed it was able to effectively alleviate the inflammation-caused damage by releasing H₂S and eliminating H₂O₂. The new protocol demonstrates its promising to achieve diagnosis and treatment of cellular inflammatory process.

Keywords: Hydrogen peroxide; Hydrogen sulfide; Inflammatory process; Two-photon; Viscosity.

MeSH terms

Fluorescence
Fluorescent Dyes*
HeLa Cells
Humans
Hydrogen Peroxide
Hydrogen Sulfide*
Inflammation / drug therapy
Viscosity

Substances

Fluorescent Dyes
Hydrogen Peroxide
Hydrogen Sulfide