Characterization and phylogenetic analysis of the complete chloroplast genome of Ilex rotunda, traditional Chinese medicine plant

Hong Chen; Xinran Chong; Yanwei Zhou; Linhe Sun; Fan Zhang; Xiaoqing Lu; Naiwei Li; Ting Zhou; Yunlong Li

doi:10.1080/23802359.2021.1967804

Characterization and phylogenetic analysis of the complete chloroplast genome of Ilex rotunda, traditional Chinese medicine plant

Mitochondrial DNA B Resour. 2021 Aug 31;6(10):2791-2792. doi: 10.1080/23802359.2021.1967804. eCollection 2021.

Authors

Hong Chen¹, Xinran Chong¹, Yanwei Zhou¹, Linhe Sun¹, Fan Zhang¹, Xiaoqing Lu¹, Naiwei Li¹, Ting Zhou¹, Yunlong Li¹

Affiliation

¹ Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.

Abstract

Ilex rotunda is a traditional Chinese medicine plant. In this study, we characterized the complete chloroplast (cp) genome sequence of I. rotunda to investigate its phylogenetic relationship. The cp genome of I. rotunda was 157,743 bp in length with 37.62% overall GC content, including a large single-copy (LSC) region of 87,060bp, a small single-copy (SSC) region of 18,432 bp, which were separated by a pair of inverted repeats (IRS) of 26,121 bp. The cp genome contained 133 genes, including 88 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on whole cp genome sequences showed that I. rotunda is closely related to I. pubescens and I. polyneura.

Keywords: Aquifoliaceae; Ilex rotunda; chloroplast genome; phylogenetic analysis.

Grants and funding

This work was financially supported by the Independent Research Project of Jiangsu province [JSPKLB202036], Forestry Science and Technology Popularization Demonstration Project of the central finance [Su(2018)TG001] and the Jiangsu Institute of Botany Talent Fund [JIBTF202110, JIBTF202111].