Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

Skadi Lau; Yue Liu; Anna Maier; Steffen Braune; Manfred Gossen; Axel T Neffe; Andreas Lendlein

doi:10.1557/s43579-021-00072-6

Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

MRS Commun. 2021;11(5):559-567. doi: 10.1557/s43579-021-00072-6. Epub 2021 Sep 7.

Authors

Skadi Lau¹, Yue Liu¹, Anna Maier¹, Steffen Braune¹, Manfred Gossen¹, Axel T Neffe¹, Andreas Lendlein^{1

2}

Affiliations

¹ Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Hereon, Teltow, Germany.
² Institute of Chemistry, University of Potsdam, Potsdam, Germany.

Abstract

In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.

Supplementary information: The online version contains supplementary material available at 10.1557/s43579-021-00072-6.

Keywords: Biomedical; Chemical composition; Polymer.