Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids

Tatiana P Fedorchuk; Inga A Kireeva; Vera K Opanasenko; Vasily V Terentyev; Natalia N Rudenko; Maria M Borisova-Mubarakshina; Boris N Ivanov

doi:10.3389/fpls.2021.662082

Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids

Front Plant Sci. 2021 Aug 26:12:662082. doi: 10.3389/fpls.2021.662082. eCollection 2021.

Authors

Tatiana P Fedorchuk¹, Inga A Kireeva², Vera K Opanasenko¹, Vasily V Terentyev¹, Natalia N Rudenko¹, Maria M Borisova-Mubarakshina¹, Boris N Ivanov¹

Affiliations

¹ Institute of Basic Biological Problems, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Russia.
² Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, Toronto, ON, Canada.

Abstract

We studied bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from leaves of Arabidopsis thaliana plants. This stimulation was not observed in thylakoids of wild-type in the presence of mafenide, a soluble carbonic anhydrase inhibitor, and was absent in thylakoids of two mutant lines lacking the gene encoding alpha carbonic anhydrase 5 (αCA5). Using mass spectrometry, we revealed the presence of αCA5 in stromal thylakoid membranes of wild-type plants. A possible mechanism of the photophosphorylation stimulation by bicarbonate that involves αCA5 is proposed.

Keywords: ATP synthase; bicarbonate; carbonic anhydrase; photophosphorylation; thylakoid membrane.