Overview of in-capillary enzymatic reactions using capillary electrophoresis

Jef Rozenski; Adissu Alemayehu Asfaw; Ann Van Schepdael

doi:10.1002/elps.202100161

Overview of in-capillary enzymatic reactions using capillary electrophoresis

Electrophoresis. 2022 Jan;43(1-2):57-73. doi: 10.1002/elps.202100161. Epub 2021 Sep 23.

Authors

Jef Rozenski¹, Adissu Alemayehu Asfaw^{2

3}, Ann Van Schepdael²

Affiliations

¹ Department ofPharmaceutical and Pharmacological Sciences, Medicinal Chemistry, Rega Institute, KU Leuven-University of Leuven, Leuven, Belgium.
² Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, KU Leuven- University of Leuven, Leuven, Belgium.
³ Department of Pharmaceutical Analysis and Quality Assurance, College of Health Sciences, School of Pharmacy, Mekelle University, Mekelle, Ethiopia.

PMID: 34510496
DOI: 10.1002/elps.202100161

Abstract

This review summarizes the research that has recently been performed on in-capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis, At-inlet and Transverse Diffusion of Laminar Flow Profiles. The main heterogeneous ones are Immobilized MicroEnzyme Reactors with enzymes grafted on either non-magnetic or magnetic particles. The overview covers the period from 2018 to early 2021. The applications range from drug discovery over natural products to food, beverage and pesticide analysis.

Keywords: Capillary electrophoresis; Enzyme; In-capillary reactions; Review.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Electrophoresis, Capillary*
Enzymes, Immobilized*

Substances

Enzymes, Immobilized